Synchrotron Radiation Infrared Spectromicroscopy: a Non- Invasive Chemical Probe for Monitoring Biogeochemical Processes
نویسندگان
چکیده
A long-standing desire in biogeochemistry is to be able to examine the cycling of elements by microorganisms as the processes are happening on surfaces of earth and environmental materials. Over the past decade, physics, engineering and instrumentation innovations have led to the introduction of synchrotron radiation-based infrared spectromicroscopy. Spatial resolutions of less than ten micrometers and photon energies of less than an electron volt make synchrotron infrared spectromicroscopy non-invasive and useful for following the course of biogeochemical processes on complex heterogeneous surfaces of earth and environmental materials. In this review, we will first briefly describe the technology, and then present several examples demonstrating its application potentials of the technology in probing and imaging biogeochemical processes.
منابع مشابه
Tracking chemical changes in a live cell: Biomedical Applications of SR- FTIR Spectromicroscopy
Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an indiv...
متن کاملSynchrotron infrared spectromicroscopy as a novel bioanalytical microprobe for individual living cells: cytotoxicity considerations.
Synchrotron radiation-based Fourier transform infrared spectromicroscopy is a newly emerging analytical tool capable of monitoring the biochemistry within an individual living mammalian cell in real time. This unique technique provides infrared (IR) spectra, hence chemical information, with high signal to noise at spatial resolutions as fine as 3-10 microm. Mid-IR photons are too low in energy ...
متن کاملSynchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres
The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide (PLGA) microsphere using synchrotron radiation-based Fourier-transform infrared spectromicroscopy (SR-FTIR). The representative infrared wavenumbers specific for protein/peptide (Exenatide) and excipient (PLGA) we...
متن کاملSynchrotron-Based FTIR Spectromicroscopy: Cytotoxicity and Heating Considerations.
Synchrotron radiation-based Fouriertransform infrared (SR-FTIR)spectromicroscopy is a newly emergingbioanalytical and imaging tool. This uniquetechnique provides mid-infrared (IR)spectra, hence chemical information, withhigh signal-to-noise at spatial resolutionsas fine as 3 to 10 microns. Thus it enablesresearchers to locate, identify, and trackspecific chemical events within anindividual livi...
متن کاملIndividual Human Cell Responses to Low Doses of Chemicals Studied by Synchrotron Infrared Spectromicroscopy
Vibrational spectroscopy, when combined with synchrotron radiation-based (SR) microscopy, is a powerful new analytical tool with high spatial resolution for detecting biochemical changes in individual living cells. In contrast to other microscopy methods that require fixing, drying, staining or labeling, SR-FTIR microscopy probes intact living cells providing a composite view of all of the mole...
متن کامل